Part:BBa_K3279000:Design

Geranylgeranyl diphosphate synthase (CrtE) from Rhodobacter sphaeroides

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 852
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 531
  Illegal SapI.rc site found at 592
  

Design Notes

There is a PstI site in the sequence, so we made a site-specific mutation to remove the illegal site.

Source

This part comes from Rhodobacter sphaeroides's genomic sequence.